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Types of microscopes and suitable applications of measuring microscopes

The main types of microscopes are distinguished based on operating principle and light source used, which in turn leads to different characteristics and applications. 

The Nikon SMZ445 is a stereo zoom microscope with dual zoom objective optics.

Distinguishing types of microscopes

The table below summarizes the major differences between common types of microscopes:

Characteristic Optical (light) microscopeElectron microscopy (EM)Fluorescence microscopeStereo microscope
Light source/rayVisible lightElectron beamShort wavelength stimulating lightNatural light/lighting
MagnificationLow to medium (maximum ~1000x – 1500x)Very high (up to millions of times)Medium to highLow to moderate (usually <100x)
ResolutionLower than EMVery high (allows to see nano structures, viruses)Better than conventional opticsMedium
Specimen typeLive or fixed, thin sampleFixed, dry, conductive sample (usually requires metal coating)Sample with fluorescent tagLarge, monolithic specimen, no slicing required
Image2D, natural color (if using light field)2D (TEM) or 3D (SEM), black and whiteGlow on dark background3D (holographic), multidimensional

Electron microscope ANMO DINO-LITE AM7915MZT ( 5Mp/ 10x to 220x )

Applications of microscopes

Applications vary depending on the type of microscope:

  • Optical microscope:
    • Biology/Medicine: Observe cells, bacteria, tissues in the laboratory and diagnose diseases.
    • Education: Teaching and basic research in schools.
  • Electron microscope (SEM, TEM):
    • Scientific research: Research on the structure of materials at the nano and virus levels.
    • Industrial: Semiconductor testing, forensic engineering, soil and rock research, materials quality control.
  • Fluorescence microscope:
    • Cell Biology/Medicine: Study of morphology, physiology, immunology, and genetics by labeling specific structures with fluorescent substances.
  • Stereo microscope:
    • Industrial: Check electronic components, circuit boards, jewelry, watch repair.
    • Biology: Analysis of larger specimens such as insects, plants, microscopic dissection.

In electronics manufacturing plants (especially for inspection of PCB boards, solder joints, components), the selection of microscopes should focus on the criteria to ensure work efficiency, precision and comfort for the operator. 

Here are the most important criteria:

1. Type of microscope (Priority 1)

The two main choices in the electronics industry are:

  • Stereo Microscope: This is the most common type.
    • Advantage: Provide photos 3D (holographic image), good depth of field, wide working distance, allowing easy rework, soldering, and component handling under glass.
    • Magnification: Typically 10x to 100x, suitable for most board inspection jobs.
  • Digital Microscope:
    • Advantage: Eliminate eyepiece, display image directly on HD screen. Great for multiple people observing, training, taking photos/videos for documentation, and working for long periods without eye strain (ergonomics).
    • Disadvantages: Usually only gives 2D images, which can be more difficult when performing operations that require depth perception (soldering, picking up components). 

2. Working Distance

This is the criterion extremely important in electronics manufacturing.

  • Working distance is the distance from the objective to the specimen (board).
  • You need enough clearance (usually >100mm) to allow room for your hands, soldering iron, tweezers, and other tools to work on the board without hitting the microscope. Stereo microscopes usually meet this criteria well. 

3. Magnification and Resolution

  • Magnification: In electronics, magnification from 10x to 100x is sufficient for most routine PCB solder, crack, and break testing jobs.
  • Resolution and optical quality: The image must be sharp and clear, especially at the edges of the field of view (choose a Plan or Semi-Plan objective if budget allows) to detect small defects.
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4. Ergonomics

Operators often sit working with microscopes for many hours at a time.

  • Binocular: More comfortable than monocles.
  • Trinocular: Allows to attach an additional camera to output to an external monitor, significantly reducing eye strain and making it convenient for recording images.
  • Viewpoint: Comfortable viewing angle (e.g. 38°) helps users maintain natural posture, reducing neck and shoulder pain. 

5. Camera and Software Integration Capability

  • Recording images, comparing with sample standards, and storing data are necessary for quality control (QC).
  • A microscope with a camera port (trinocular type) or a digital microscope with accompanying software will greatly assist in this work.

To contact Semiki Measuring Equipment Co., Ltd. for advice and purchase of suitable microscopes, you can use the following methods: 

Quick contact information

Contact via website

  • Website: www.semiki.com
  • You can access the “Contact” or “Request a quote” section on the home page to send information directly. 

When contacting, please prepare information such as usage needs (electronic component testing, solder joint testing, etc.), desired magnification level and other technical requirements for the most accurate and effective consultation.

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